LOCUS       V005577                 8628 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005577
VERSION     V005577
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8628)
  AUTHORS   Giroglou T, Cinatl J Jr., Rabenau H, Drosten C, Schwalbe H, Doerr
            HW, von Laer D.
  TITLE     Retroviral vectors pseudotyped with severe acute respiratory
            syndrome coronavirus S protein
  JOURNAL   J. Virol. 78 (17), 9007-9015 (2004)
   PUBMED   15308697
REFERENCE   2  (bases 1 to 8628)
  AUTHORS   Giroglou T.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2004) Applied Virology and Genetherapy,
            Georg-Speyer-Haus, Paul-Ehrlich-Str. 42-44, Frankfurt 60596, Germany
REFERENCE   3  (bases 1 to 8628)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8628)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Virol.";
            date: "2004"; volume: "78"; issue: "17"; pages: "9007-9015"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-MAR-2004) Applied Virology and Genetherapy, Georg-Speyer-Haus,
            Paul-Ehrlich-Str. 42-44, Frankfurt 60596, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8628
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        83..462
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        463..666
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          778..1350
                     /label="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     CDS             1420..5184
                     /gene="S"
                     /label="Spike glycoprotein"
                     /note="Spike glycoprotein from Severe acute respiratory
                     syndrome coronavirus. Accession#: P59594"
     polyA_signal    5384..5439
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(5797..5813)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5821..5837)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5845..5875)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5890..5911)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6199..6787)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6961..7818)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7819..7923)
                     /label="AmpR promoter"
     rep_origin      complement(7949..8404)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     8546..8562
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        8572..8590
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagcttggcc cattgcatac gttgtatcca tatcataata tgtacattta tattggctca
       61 tgtccaacat taccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt
      121 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      181 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      241 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      301 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      361 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      421 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      481 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      541 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      601 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      661 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
      721 catagaagac accgggaccg atccagcctc cggtcgaccg atcctgagaa cttcagggtg
      781 agtttgggga cccttgattg ttctttcttt ttcgctattg taaaattcat gttatatgga
      841 gggggcaaag ttttcagggt gttgtttaga atgggaagat gtcccttgta tcaccatgga
      901 ccctcatgat aattttgttt ctttcacttt ctactctgtt gacaaccatt gtctcctctt
      961 attttctttt cattttctgt aactttttcg ttaaacttta gcttgcattt gtaacgaatt
     1021 tttaaattca cttttgttta tttgtcagat tgtaagtact ttctctaatc actttttttt
     1081 caaggcaatc agggtatatt atattgtact tcagcacagt tttagagaac aattgttata
     1141 attaaatgat aaggtagaat atttctgcat ataaattctg gctggcgtgg aaatattctt
     1201 attggtagaa acaactacac cctggtcatc atcctgcctt tctctttatg gttacaatga
     1261 tatacactgt ttgagatgag gataaaatac tctgagtcca aaccgggccc ctctgctaac
     1321 catgttcatg ccttcttctc tttcctacag ctcctgggca acgtgctggt tgttgtgctg
     1381 tctcatcatt ttggcaaaga attcctcgac ggatccacca tgtttatttt cttattattt
     1441 cttactctca ctagtggtag tgaccttgac cggtgcacca cttttgatga tgttcaagct
     1501 cctaattaca ctcaacatac ttcatctatg aggggggttt actatcctga tgaaattttt
     1561 agatcagaca ctctttattt aactcaggat ttatttcttc cattttattc taatgttaca
     1621 gggtttcata ctattaatca tacgtttggc aaccctgtca taccttttaa ggatggtatt
     1681 tattttgctg ccacagagaa atcaaatgtt gtccgtggtt gggtttttgg ttctaccatg
     1741 aacaacaagt cacagtcggt gattattatt aacaattcta ctaatgttgt tatacgagca
     1801 tgtaactttg aattgtgtga caaccctttc tttgctgttt ctaaacccat gggtacacag
     1861 acacatacta tgatattcga taatgcattt aattgcactt tcgagtacat atctgatgcc
     1921 ttttcgcttg atgtttcaga aaagtcaggt aattttaaac acttacgaga gtttgtgttt
     1981 aaaaataaag atgggtttct ctatgtttat aagggctatc aacctataga tgtagttcgt
     2041 gatctacctt ctggttttaa cactttgaaa cctattttta agttgcctct tggtattaac
     2101 attacaaatt ttagagccat tcttacagcc ttttcacctg ctcaagacat ttggggcacg
     2161 tcagctgcag cctattttgt tggctattta aagccaacta catttatgct caagtatgat
     2221 gaaaatggta caatcacaga tgctgttgat tgctctcaaa atccacttgc tgaactcaaa
     2281 tgctctgtta agagctttga gattgacaaa ggaatttacc agacctctaa tttcagggtt
     2341 gttccctcag gagatgttgt gagattccct aatattacaa acttgtgtcc ttttggagag
     2401 gtttttaatg ctactaaatt cccttctgtc tatgcatggg agagaaaaaa aatttctaat
     2461 tgtgttgctg attactctgt gctctacaac tcaacatttt tttcaacctt taagtgctat
     2521 ggcgtttctg ccactaagtt gaatgatctt tgcttctcca atgtctatgc agattctttt
     2581 gtagtcaagg gagatgatgt aagacaaata gcgccaggac aaactggtgt tattgctgat
     2641 tataattata aattgccaga tgatttcatg ggttgtgtcc ttgcttggaa tactaggaac
     2701 attgatgcta cttcaactgg taattataat tataaatata ggtatcttag acatggcaag
     2761 cttaggccct ttgagagaga catatctaat gtgcctttct cccccgatgg caaaccttgc
     2821 accccacctg ctcttaattg ttattggcca ttaaatgatt atggttttta caccactact
     2881 ggcattggct accaacctta cagagttgta gtactttctt ttgaactttt aaatgcaccg
     2941 gccacggttt gtggaccaaa attatccact gaccttatta agaaccagtg tgtcaatttt
     3001 aattttaatg gactcactgg tactggtgtg ttaactcctt cttcaaagag atttcaacca
     3061 tttcaacaat ttggccgtga tgtttctgat ttcactgatt ccgttcgaga tcctaaaaca
     3121 tctgaaatat tagacatttc accttgctct tttgggggtg taagtgtaat tacacctgga
     3181 acaaatgctt catctgaagt tgctgttcta tatcaagatg ttaactgcac tgatgtttct
     3241 acagcaattc atgcagatca actcacacca gcttggcgca tatactctac tggaaacaat
     3301 gtattccaga ctcaagcagg ctgtcttata ggagctgagt atgtcgacac ttcttatgag
     3361 tgcgacattc ctattggagc tggcatttgt gctagttacc atacagtttc tttattacgt
     3421 agtactagcc aaaaatctat tgtggcttat actatgtctt taggtgctga tagttcaatt
     3481 gcttactcta ataacaccat tgctatacct actaactttt caattagcat tactacagaa
     3541 gtaatgcctg tttctatggc taaaacctcc gtagattgta atatgtacat ctgcggagat
     3601 tctactgaat gtgctaattt gcttctccaa tatggtagct tttgcacaca actaaatcgt
     3661 gcactctcag gtattgctgc tgaacaggat cgcaacacac gtgaagtgtt cgctcaagtc
     3721 aaacaaatgt acaaaacccc aactttgaaa tattttggtg gttttaattt ttcacaaata
     3781 ttacctgacc ctctaaagcc aactaagagg tcttttattg aggacttgct ctttaataag
     3841 gtgacactcg ctgatgctgg cttcatgaag caatatggcg aatgcctagg tgatattaat
     3901 gctagagatc tcatttgtgc gcagaagttc aatggactta cagtgttgcc acctctgctc
     3961 actgatgata tgattgctgc ctacactgct gctctagtta gtggtactgc cactgctgga
     4021 tggacatttg gtgctggcgc tgctcttcaa ataccttttg ctatgcaaat ggcatatagg
     4081 ttcaatggca ttggagttac ccaaaatgtt ctctatgaga accaaaaaca aatcgccaac
     4141 caatttaaca aggcgattag tcaaattcaa gaatcactta caacaacatc aactgcattg
     4201 ggcaagctgc aagacgttgt taaccagaat gctcaagcat taaacacact tgttaaacaa
     4261 cttagctcta attttggtgc aatttcaagt gtgctaaatg atatcctttc gcgacttgat
     4321 aaagtcgagg cggaggtaca aattgacagg ttaattacag gcagacttca aagccttcaa
     4381 acctatgtaa cacaacaact aatcagggct gctgaaatca gggcttctgc taatcttgct
     4441 gctactaaaa tgtctgagtg tgttcttgga caatcaaaaa gagttgactt ttgtggaaag
     4501 ggctaccacc ttatgtcctt cccacaagca gccccgcatg gtgttgtctt cctacatgtc
     4561 acgtatgtgc catcccagga gaggaacttc accacagcgc cagcaatttg tcatgaaggc
     4621 aaagcatact tccctcgtga aggtgttttt gtgtttaatg gcacttcttg gtttattaca
     4681 cagaggaact tcttttctcc acaaataatt actacagaca atacatttgt ctcaggaaat
     4741 tgtgatgtcg ttattggcat cattaacaac acagtttatg atcctctgca acctgagctt
     4801 gactcattca aagaagagct ggacaagtac ttcaaaaatc atacatcacc agatgttgat
     4861 tttggcgaca tttcaggcat taacgcttct gtcgtcaaca ttcaaaaaga aattgaccgc
     4921 ctcaatgagg tcgctaaaaa tttaaatgaa tcactcattg accttcaaga attgggaaaa
     4981 tatgagcaat atattaaatg gccttggtat gtttggctcg gcttcattgc tggactaatt
     5041 gccatcgtca tggttacaat cttgctttgt tgcatgacta gttgttgcag ttgcctcaag
     5101 ggtgcatgct cttgtggttc ttgctgcaag tttgatgagg atgactctga gccagttctc
     5161 aagggtgtca aattacatta cacataaggt accctcgaga tcgatgatat ccccgggctg
     5221 cagcgatccg tcgaggaatt cactcctcag gtgcaggctg cctatcagaa ggtggtggct
     5281 ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc tgccaaaaat
     5341 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt
     5401 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg
     5461 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat
     5521 atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc
     5581 cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata
     5641 ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta
     5701 gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg
     5761 agatccctcg acggatcggc cgcaattcgt aatcatgtca tagctgtttc ctgtgtgaaa
     5821 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     5881 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     5941 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     6001 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     6061 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     6121 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     6181 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     6241 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     6301 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     6361 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     6421 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     6481 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     6541 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     6601 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     6661 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     6721 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     6781 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     6841 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     6901 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     6961 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     7021 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     7081 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     7141 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     7201 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     7261 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     7321 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     7381 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     7441 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     7501 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     7561 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     7621 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     7681 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     7741 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     7801 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     7861 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     7921 gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc
     7981 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     8041 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     8101 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     8161 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     8221 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     8281 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     8341 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     8401 gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     8461 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     8521 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac
     8581 tcactatagg gcgaattgga gctccaccgc ggtggcggcc gctctaga
//
